Research Article

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2014, 7(5): 658–669

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https://doi.org/10.1007/s12274-014-0420-4

AS1411 aptamer-conjugated Gd2O3:Eu nanoparticles for target-specific computed tomography/magnetic resonance/fluorescence molecular imaging

Tsungrong Kuo1,2, Weiyun Lai3,4, Chenghung Li1, Yanjhan Wun1, Huancheng Chang2, Jinnshiun Chen5, Panchyr Yang6, and Chiachun Chen1,2 (*)

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1 Department of Chemistry, “National Taiwan Normal University”, Taipei 116, Taiwan, China
2 Institute of Atomic and Molecular Science, “Academia Sinica”, Taipei 106, Taiwan, China
3 Molecular Medicine Program, Taiwan International Graduate Program, Institute of Biomedical Sciences, “Academia Sinica”, Taipei 115, Taiwan, China
4 Institute of Biochemistry and Molecular Biology, School of Life Sciences, “National Yang-Ming University”, Taipei 112, Taiwan, China
5 Division of Colorectal Surgery, Chang Gung Memorial Hospital, Chang Gung University, Taoyuan 333, Taiwan, China
6 College of Medicine, “National Taiwan University”, Taipei 106, Taiwan, China

Keywords: aptamer, contrast agent, Gd2O3 nanoparticles, molecular imaging, nanoparticles synthesis
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  • Abstract
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Europium-doped gadolinium oxide (Gd2O3:Eu) nanoparticles have been synthesized, and then their surfaces have been conjugated with nucleolintargeted AS1411 aptamer to form functionalized target-specific Gd2O3:Eu nanoparticles (A-GdO:Eu nanoparticles). The A-GdO:Eu nanoparticles present strong fluorescence in the visible range, high magnetic susceptibility, X-ray attenuation and good biocompatibility. The A-GdO:Eu nanoparticles have been applied to test molecular expression of nucleolin highly expressed CL1-5 lung cancer cells under a confocal microscope. Fluorescence imaging clearly reveals that the nanoparticles can be applied as fluorescent tags for cancer-targeting molecular imaging. Furthermore, taking together their excellent T1 contrast and strong computed tomography (CT) signal, the A-GdO:Eu nanoparticles demonstrate a great capability for use as a dual modality contrast agent for CT and magnetic resonance (MR) molecular imaging. Animal experiments also show that the A-GdO:Eu nanoparticles are able to contrast the tissues of BALB/c mice using CT modality. Moreover, the obvious red fluorescence of A-GdO:Eu nanoparticles can be visualized in a tumor by the naked eye. Overall, our results demonstrate that the A-GdO:Eu nanoparticles can not only serve as new medical contrast agents but also as intraoperative fluorescence imaging probes for guided surgery in the near future.
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AS1411 aptamer-conjugated Gd2O3:Eu nanoparticles for target-specific computed tomography/magnetic resonance/fluorescence molecular imaging. Nano Res. 2014, 7(5): 658–669 https://doi.org/10.1007/s12274-014-0420-4

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