Research Article

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2015, 8(3): 774–789

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https://doi.org/10.1007/s12274-014-0560-6

Structural analysis of a nanoparticle containing a lipid bilayer used for detergent-free extraction of membrane proteins

Mohammed Jamshad1,§, Vinciane Grimard2,§, Ilaria Idini3,§, Tim J. Knowles4,§, Miriam R. Dowle5, Naomi Schofield1, Pooja Sridhar4, Yupin Lin1, Rachael Finka1, Mark Wheatley1, Owen R. T. Thomas6, Richard E. Palmer5, Michael Overduin4, Cédric Govaerts2, Jean-Marie Ruysschaert2, Karen J. Edler3, and Tim R. Dafforn1 (*)

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1 School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
2 Université Libre de Bruxelles, SFMB - CP206/2, Bd. du Triomphe, Accès 2 1050, Bruxelles, Belgium
3 Department of Chemistry, University of Bath, Claverton Down, Bath, BA2 7AY, UK
4 School of Cancer Studies, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
5 Nanoscale Physics Research Laboratory and PSIBS, School of Physics and Astronomy, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
6 School of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
§ These authors contributed equally to the work.

Keywords: nanoparticles, lipid, polymer, membrane proteins, structure, detergent
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  • Abstract
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In the past few years there has been a growth in the use of nanoparticles for stabilizing lipid membranes that contain embedded proteins. These bionanoparticles provide a solution to the challenging problem of membrane protein isolation by maintaining a lipid bilayer essential to protein integrity and activity. We have previously described the use of an amphipathic polymer (poly(styrene-co-maleic acid), SMA) to produce discoidal nanoparticles with a lipid bilayer core containing the embedded protein. However the structure of the nanoparticle itself has not yet been determined. This leaves a major gap in understanding how the SMA stabilizes the encapsulated bilayer and how the bilayer relates physically and structurally to an unencapsulated lipid bilayer. In this paper we address this issue by describing the structure of the SMA lipid particle (SMALP) using data from small angle neutron scattering (SANS), electron microscopy (EM), attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), differential scanning calorimetry (DSC) and nuclear magnetic resonance spectroscopy (NMR). We show that the particle is disc shaped containing a polymer “bracelet” encircling the lipid bilayer. The structure and orientation of the individual components within the bilayer and polymer are determined showing that styrene moieties within SMA intercalate between the lipid acyl chains. The dimensions of the encapsulated bilayer are also determined and match those measured for a natural membrane. Taken together, the description of the structure of the SMALP forms the foundation for future development and applications of SMALPs in membrane protein production and analysis.
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Structural analysis of a nanoparticle containing a lipid bilayer used for detergent-free extraction of membrane proteins. Nano Res. 2015, 8(3): 774–789 https://doi.org/10.1007/s12274-014-0560-6

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