Research Article


2019, 12(2): 413–420


SERS-based lateral flow immunoassay of troponin I by using gapenhanced Raman tags

Boris N. Khlebtsov1,2 (*), Daniil N. Bratashov2, Nadezhda A. Byzova3, Boris B. Dzantiev3, and Nikolai G. Khlebtsov1,2 (*)

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1 Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov 410049, Russia
2 Saratov State University, Saratov 410012, Russia
3 A. N. Bach Institute of Biochemistry, Research Centre of Biotechnology, Russian Academy of Sciences, Moscow 119071, Russia

Keywords: SERS, lateral flow immunoassay, Au core/shell nanorods, gap-enhanced Raman tags, cardiac Troponin I
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The lateral flow immunoassay (LFIA) has emerged as a powerful tool for rapid screening owing to its simplicity and flexibility for detection of various biomarkers. However, conventional LFIA strips have several disadvantages, including limits in quantitative analysis and low sensitivity. Here we developed a novel surface-enhanced Raman scattering LFIA based on nonspherical gap-enhanced Raman tags (GERTs), with Raman molecules (RMs) embedded in a 1-nm gap between Au nanorod core and Au shell. Such tags have a strong and uniform SERS response, an order of magnitude higher than that of other common SERS tags such as Au nanorods, nanostars, Au nanoshells with surface-adsorbed RMs, or spherical GERTs with embedded RMs. The feasibility of the tags was demonstrated by the semiquantitative and sensitive detection of the heart disease biomarker cardiac troponin I (cTnI). GERTs were conjugated with monoclonal antibodies and used for LFIA in the same way as ordinary functionalized colloidal gold. The presence of the target antigen, cTnI, was identified by Raman microscopy mapping of the test zone. With the SERS-based LFIA, the limit of cTnI detection was about 0.1 ng/mL. This value is within the diagnostic range of cTnI in the blood serum of patients with heart infarction and is 30 times lower than that of the colorimetric LFIA test using the same antibodies and either GERTs or colloidal gold as labels.
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SERS-based lateral flow immunoassay of troponin I by using gapenhanced Raman tags. Nano Res. 2019, 12(2): 413–420

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