Research Article


2019, 12(4): 749–757


Infrared fluorescence imaging of infarcted hearts with Ag2S nanodots

Dirk H. Ortgies1,2, ngel Luis García-Villalón3, Miriam Granado3, Sara Amor3, Emma Martín Rodríguez1,4, Harrisson D.A. Santos2,5, Jingke Yao2, Jorge Rubio-Retama6, and Daniel Jaque1,2 (*)

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1 Nanobiology Group, Instituto Ramón y Cajal de Investigación Sanitaria, IRYCIS, Ctra. Colmenar km. 9.100, Madrid 28034, Spain
2 Fluorescence Imaging Group, Departamento de Física de Materiales – Facultad de Ciencias, Universidad Autónoma de Madrid, C/ Francisco Tomás y Valiente 7, Madrid 28049, Spain
3 Fluorescence Imaging Group, Departamento de Fisiología – Facultad de Medicina, Avda. Arzobispo Morcillo 2, Universidad Autónoma de Madrid, Madrid 28029, Spain
4 Fluorescence Imaging Group, Departamento de Física Aplicada – Facultad de Ciencias, Universidad Autonoma de Madrid, C/ Francisco Tomás y Valiente 7, Madrid 28049, Spain
5 Grupo de Nano-Fotnica e Imagens, Instituto de Física, Universidade Federal de Alagoas, Maceió-AL 57072-900, Brazil
6 Departamento de Química Física en Ciencias Farmacéuticas, Facultad de Farmacia, Plaza de Ramón y Cajal, s/n, Universidad Complutense de Madrid, Madrid 28040, Spain

Keywords: infrared imaging, myocardial infarct, Ag2S, nanodots, biological windows, Langendorff heart
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Ag2S nanodots have already been demonstrated as promising near-infrared (NIR-II, 1.0–1.45 μm) emitting nanoprobes with low toxicity, high penetration and high resolution for in vivo imaging of, for example, tumors and vasculature. In this work, we have systematically investigated the potential application of functionalized Ag2S nanodots for accurate imaging of damaged myocardium tissues after a myocardial infarction induced by either partial or global ischemia. Ag2S nanodots surface-functionalized with the angiotensin II peptide (ATII) have shown over 10-fold enhanced binding efficiency to damaged tissues than non-specifically (PEG) functionalized Ag2S nanodots due to their interaction with the upregulated angiotensin II receptor type I (AT1R). It is demonstrated how the NIR-II images generated by ATII-functionalized Ag2S nanodots contain valuable information about the location and extension of damaged tissue in the myocardium allowing for a proper identification of the occluded artery as well as an indirect evaluation of the damage level. The potential application of Ag2S nanodots in the near future for in vivo imaging of myocardial infarction was also corroborated by performing proof of concept whole body imaging experiments.
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Infrared fluorescence imaging of infarcted hearts with Ag2S nanodots. Nano Res. 2019, 12(4): 749–757

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