Research Article


2021, 14(11): 3840–3847


Long-term fundus fluorescence angiography and real-time diagnosis of retinal diseases in non-human primate-animal models

Miaomiao Tang1,§, Lu Zhang1,§, Bin Song1,§, Xiaoyuan Ji1, Chenyu Wang1, Houyu Wang1, Hua Xu2, Yuanyuan Su1,3, and Yao He1 (✉)

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1 Laboratory of Nanoscale Biochemical Analysis, Institute of Functional Nano and Soft Materials (FUNSOM) and Collaborative Innovation
Center of Suzhou Nano Science and Technology, Soochow University, Suzhou 215123, China
2 Department of Ophthalmology, Children’s Hospital of Soochow University, Soochow University, Suzhou 215123, China
3 Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Medical Research Center, Sun Yat-Sen Memorial
Hospital, Sun Yat-Sen University, Guangzhou 510120, China
§ Miaomiao Tang, Lu Zhang, and Bin Song contributed equally to this work.

Keywords: fluorescent silicon nanoparticles, long-term, real-time, bioimaging, retinal diseases
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  • Abstract
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Fluorescein angiography (FA) is a standard imaging modality for evaluating vascular abnormalities in retina-related diseases, which is recognized as the major cause of vision loss. Long-term and real-time fundus angiography is of great importance in preclinical research, nevertheless remaining big challenges up to present. In this study, we demonstrate that long-term fluorescence imaging of retinal vessels is enabled through a kind of fluorescent nanoagents, which is made of small-sized (hydrodynamic diameter: ~ 3 nm) silicon nanoparticles (SiNPs) featuring strong fluorescence, robust photostability, lengthened blood residency and negligible toxicity. In particular, the presented SiNPs-based nanoagents are capable of imaging retinal capillaries in ~ 10 min, which is around 10-fold longer than that (~ 1 min) of fluorescein sodium (FS, known as the most widely used contrast agents for FA in clinic). Taking cynomolgus macaques as non-human primate-animal model, we further demonstrate the feasibility of real-time diagnosis of retinal diseases (e.g., age-related macular degeneration (AMD)) through dynamic monitoring of vascular dysfunction.
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Long-term fundus fluorescence angiography and real-time diagnosis of retinal diseases in non-human primate-animal models. Nano Res. 2021, 14(11): 3840–3847

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