Research Article


2021, 14(9): 3309–3318


Highly efficient artificial blood coagulation shortcut confined on Ca-zeolite surface

Lisha Yu1,§, Bin Yu1,§, Hao Chen1, Xiaoqiang Shang1, Min He2, Mengchi Lin1, Dan Li1, Wenzhao Zhang1, Zhengzhong Kang1, Jiachen Li1, Fangjun Wang2, Liping Xiao1, Qi Wang1 (✉), and Jie Fan1 (✉)

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1 Department of Chemistry, Zhejiang University, Hangzhou 310027, China
2 CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
§ Lisha Yu and Bin Yu contributed equally to this work.

Keywords: surface-confined, Ca-zeolite, blood coagulation, trypsin
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It is challenging to develop an in vitro catalytic system to conduct natural surface-confined enzymatic reactions in a stable, efficient, and spatially defined manner. Here, we report that an artificial catalyst, which composes of trypsin and a calcium ion exchanged zeolite Y (trypsin/CaY), is capable of conducting surface-confined thrombin generation, and then constructs an artificial shortcut for classic, natural and complex blood coagulation cascade. The Ca2+ within the microporous cages play a key role in trypsin/CaY hybrid through tuning the bio-inorganic interaction and spatial orientation of the protease, which allows trypsin/CaY to display greatly enhanced catalytic performance in coagulation process. The in vivo efficiency of the artificial coagulation shortcut is further confirmed in massive bleeding and hemophilia animal models. Rapid hemostasis is achieved by trypsin/CaY hybrid in a hemophilia A mice tail bleeding model, where natural clotting system fails in response to bleeding event due to factor VIII deficiency. In a rabbit lethal femoral artery injury model, the blood loss of the artificial catalyst is decreased by 4–7 fold when compared to state-of-art clay- or zeolite-based topical agents.
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Highly efficient artificial blood coagulation shortcut confined on Ca-zeolite surface. Nano Res. 2021, 14(9): 3309–3318

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